Human Tissue EngineeringIdentifying activated micro RNAs
A key challenge in glioblastoma biology and relevant tumor environment modeling lies in understanding of the cellular interactions occuring within the context of a 3-dimensional (3-D) human tissue microenvironment.
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Figure 1: hESC (or iPSC) colonies were detached with accutase and cultured in suspension in ultralow attachment plates for 4-6 days in neural induction medium. After four weeks, about 30 hESCderived clusters were plated on a hydrophilic polytetrafluoroethylene(PTFE) membrane deposited on a Millicell-CM (0.4mm) culture plate insert designed for 6-well plates. One milliliter of neural induction medium was added to each well under the membrane insert for differentiation during 4-6 weeks. Glioma-initiating cells (GICs) can be transduced for imaging (Tomato Fluorescent Protein, TFP) and apply on the top of the tissue for 2 weeks of co-culture.