Real-Time PCRYet More Worlds to Conquer
The polymerase chain reaction (PCR), invented about three decades ago, soon entered mainstream use thanks to an ongoing series of refinements. One particularly important refinement, introduced about two decades ago, is the “real time” quantification of DNA.
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Absolute quantification by digital PCR is made possible by instruments that run thousands of individual reactions in parallel. One such instrument, the QuantStudio™ 3D System from Life Technologies, uses a sealed chip technology to streamline workflow. The instrument can generate up to 20,000 data points and enable more controlled sample loading and less reaction dropout than droplet-based approaches.