Liquid Chromatography-Mass Spectrometry Metabolic and Lipidomic Sample PreparationWorkflow for Suspension-Cultured Mammalian Cells using Jurkat T lymphocyte Cells
Metabolomics is the comprehensive study of metabolism as it pertains to an organism or biological system. Lipidomics, a subset discipline of metabolomics, encompasses the study of cellular lipid functions: including pathways, networks, and interactions.
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Figure 1: Peak area of endogenous metabolites and spiked internal standard metabolite mix for each rinsing solution (0.3% ammonium formate, 0.3% ammonium acetate, 0.9% NaCl, 1 M PBS, 100 mM HEPES). The error bars correspond to the standard deviation of the mean for the triplicate samples from each rinsing solution.