Liquid Chromatography-Mass Spectrometry Metabolic and Lipidomic Sample PreparationWorkflow for Suspension-Cultured Mammalian Cells using Jurkat T lymphocyte Cells
Metabolomics is the comprehensive study of metabolism as it pertains to an organism or biological system. Lipidomics, a subset discipline of metabolomics, encompasses the study of cellular lipid functions: including pathways, networks, and interactions.
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Figure 3. [A] Peak area of exogenous lipid internal standards for each lipid extraction method (Folch, Bligh-Dyer, Acidified Bligh-Dyer, Matyash). [B] Peak area of endogenous lipids for each extraction method. Error bars correspond to the standard deviation of the mean for triplicate samples from each extraction solvent system.