Microinjection in Zebrafish and Medaka

The aim of the course is to provide participants with hands-on tutorials and the theory behind microinjection into medaka and zebrafish. In addition, participants will learn to design CRISPR constructs.

• Microinjection of lineage tracers into one-cell stage embryos
• Co-injection of DNA and mRNA into one-cell stage embryos
• Microinjection of CRISPR/Cas constructs
• Targeted inactivation of pigment loci or genes of interest provided by applicants (medaka and zebrafish)
• Homology directed repair in fish
• Screening by imaging
• High throughput, high content screening for successful events
• Molecular validation of Crispr/Cas9 mediated indel formation

Veranstalter:

Eppendorf AG
Barkhausenweg 1
22339 Hamburg

Tel: + 49 40 / 538 01 361
Fax: + 49 40 / 538 01 840
E-Mail: [email protected]