Ernst Ruska Prize 2017Cryo-EM structure of haemoglobin at 3.2 Å determined with the Volta phase plate
With the advent of direct electron detectors, the perspectives of cryo-electron microscopy (cryo-EM) have changed in a profound way.
Seite [7 von 7]
 | | | Bild 7 von 7 | | | |
Figure 6. In-focus phase plate imaging of 64 kDa Hgb. (a) Electron micrograph of metHgb recorded at ∼ 20 nm underfocus with the Volta phase plate (VPP) (scale bar, 50 nm). (b) Power spectrum of the image in a, featuring continuous signal without CTF oscillations across the frequency spectrum and visibility of the amorphous ice ring at 3.7 Å. (c) Reconstructed 3D electron scattering potential map and fitted model of Hgb from crystal structure PDB-1A9W.