Liquid Chromatography-Mass Spectrometry Metabolic and Lipidomic Sample PreparationWorkflow for Suspension-Cultured Mammalian Cells using Jurkat T lymphocyte Cells
Metabolomics is the comprehensive study of metabolism as it pertains to an organism or biological system. Lipidomics, a subset discipline of metabolomics, encompasses the study of cellular lipid functions: including pathways, networks, and interactions.
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Figure 2. Chromatogram overlay showing the ion intensity of metabolites. [A] Total Ion Chromatogram for Jurkat cells rinsed with either 0.3% ammonium formate or 0.3% ammonium acetate. [B] Total Ion Chromatogram for 6-8 min. shows an increased peak intensity for the caffeine-d3 and tryptophan-d3 internal standards for cells rinsed with 0.3% ammonium formate. Retention time reproducibility for internal standards, caffeine-d3 and tryptophan-d3 was 0.3 seconds over the entire chromatographic run. Peak Area reproducibility for internal standards, caffeine-d3 and tryptophan-d3 was 3.6 %CV and 5.8 %CV.