RNA-seqBioinformatics Tools
In recent years, RNA-seq has emerged as a powerful technology in estimation of gene or transcript expression. ‘Union-exon’ and transcript based approaches are widely used in gene quantification.
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Figure 3: Diagram of the complexity of gene structures. Multiple isoforms generated by alternative splicing, together with overlapping genes, make accurate quantification at the transcript level difficult. Assigning ambiguous reads in overlapping regions to its true origin is hard. Genes on the plus strand are colored in blue while genes on the minus strand in green.