Immunohistochemistryantibody recovery and reuse in SNAP i.d.® 2.0 system
Immunohistochemistry (IHC) is a biochemical method used for the detection, localization and identification of biomarkers in tissue samples. Although IHC is used worldwide and many automated systems exist for handling large numbers of slides.
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3: Anti-amyloid ß: one working solution used four times for four different slides. Amyloid ß, a peptide considered one of the major constituents of the plaques in Alzheimer’s disease, was detected in FFPE human Alzheimer’s disease brain tissue slides that were HIER pretreated. The mouse monoclonal anti-amyloid ß clone W0-2 was diluted 1:500 in TBS-T and incubated for 1 hr. Strong dark staining in the area where amyloid ß had been deposited was detected using both the manual IHC method and the SNAP i.d.® system, even after recovery and reuse of the antibody 3 times in a period of a week, with a volumetric recovery of more than 90 %. A no-primary antibody control is shown at bottom left.