Cell Damage and DiseaseDouble-Stranded DNA Damage Assessed with Raman Spectroscopy
Double stranded DNA breaks [DSBs] and subsequent repair can correct DNA damage or may mistakenly cause mutations leading to cell damage and disease.
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Figure 1: Raman spectra for plasmid pBS KS+, single and double digests and double strand breakage. There are single EcoR1 and Xmn1 restriction sites in the pBS KS+ plasmid. Enhanced intensities of restricted DNA were seen at 880, 1044, 1084, 1456, cm-1 (and the 1485/1456 ratio, not shown). We include the raw water/steel measurements, subsequently subtracted from each sample Raman scan.