Immunohistochemistryantibody recovery and reuse in SNAP i.d.® 2.0 system
Immunohistochemistry (IHC) is a biochemical method used for the detection, localization and identification of biomarkers in tissue samples. Although IHC is used worldwide and many automated systems exist for handling large numbers of slides.
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1: Antibody recovery and reuse in vacuum-driven IHC using the SNAP i.d.® 2.0 IHC system. Deparaffinization, HIER, dehydration and clearing of the slides were performed outside the SNAP i.d.® 2.0 IHC system. Blocking, antibody incubation and staining were performed in the SNAP i.d.® 2.0 IHC system after assembling the slides in the slide holder. Using the the SNAP i.d.® 2.0 system permits reduced slide manipulation, does not require the use of a Pap pen, and the vacuum system is likely to increase the efficiency with which solutions are removed and tissue is efficiently washed. Antibodies, or any other reagent, can be recovered with a 1 mL pipette tip through the injector recovery port, and stored for future reuse.