Immunohistochemistryantibody recovery and reuse in SNAP i.d.® 2.0 system
Immunohistochemistry (IHC) is a biochemical method used for the detection, localization and identification of biomarkers in tissue samples. Although IHC is used worldwide and many automated systems exist for handling large numbers of slides.
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2: Anti-aquaporin: one working solution used six times for six different slides. Detection of Aquaporin 1, in FFPE human kidney tissue. Comparison of samples processed by the manual protocol (control I) vs. the SNAP i.d.® 2.0 system demonstrated that 600 μL of rabbit polyclonal anti Aquaporin-1 diluted 1:2000 in TBS-T was sufficient to probe 6 human kidney slides after the antibody was recovered and reused 5 times over a period of 48 days, with antibody and sample storage at 4 °C. Volumetric recovery analyzed by mass balance revealed a recovery of more than 80 % after the 5th cycle. When primary antibody was omitted from the procedures, the slides showed no staining, indicating that the positive signal in the test slides was not due to nonspecific signals from the immunodetection reagents.