EAEC Under StressLoss of Biofilm Formation
Enteroaggregative Escherichia coli is an emerging foodborne pathogen as a significant diarrheal pathogen in multiple population groups. However, the associated phenotypic changes by which EAEC bacteria survive acid conditions, is not completely understood.
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Figure 1: Growth analysis of EAEC (T8) under acid stress. EAEC (T8) grown overnight at pH 7.4 in LB was subcultured (1:500) in minimal media (M9) with diff erent pH as described in experimental Procedures. After every 1h, cell count was measured at 600 nm in spectrophotometer at diff erent pH. (A) M9 media only. (B) M9 media with supplement lysine (0.12%). (C) M9 media with supplement arginine (0.12%). (D) FACS analysis for the adherent cell survival at various pH (in vitro) on HEp-2 cells by propidium iodide exclusion assay. Data represent the mean ± S.D. of three experiments.