Immunohistochemistryantibody recovery and reuse in SNAP i.d.® 2.0 system
Immunohistochemistry (IHC) is a biochemical method used for the detection, localization and identification of biomarkers in tissue samples. Although IHC is used worldwide and many automated systems exist for handling large numbers of slides.
Seite [4 von 6]
 | | | Bild 4 von 6 | | | |
Table 1: Antibody volumetric recovery after each round of use. The antibody recovered from the SNAP i.d.® 2.0 IHC slide holder using a 1 mL pipette tip was transferred to a microcentrifuge tube and measured by weighing using an analytical balance. Antibody working solutions were kept at 4 °C for periods of time ranging from 7 to 48 days. Percent recovery was higher than 80 % even after 48 days of storage and transfer. Volume losses could be attributed to evaporation and the transfer process from pipette tip/slide holder/microcentrifuge tube.